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Merck

04-721

Anti-Dicer1 Antibody, clone 5D12.2

clone 5D12.2, Upstate®, from mouse

Synonyma:

DCR1, Dicer, HERNA, KIAA0928

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O této položce

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5D12.2, monoclonal
Application:
WB
Citations:
5

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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D12.2, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_085124.2

UniProt accession no.

Q9UPY3

shipped in

ambient

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... DICER1(23405)

Související kategorie

General description

220 kDa
RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1

Application

Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)
Western blot

Biochem/physiol Actions

Human Dicer1

Physical form

Format: Purified
Protein G Chromatography

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Analysis Note

Routinely evaluated by western blot on lysates from HeLa nuclear extracts.

Other Notes

Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Skladovací třída

12 - Non Combustible Liquids

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Jiandong Shi et al.
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Chatla Srinivas et al.
PloS one, 10(11), e0142006-e0142006 (2015-11-10)
Tumor microenvironment play role in angiogenesis and carcinogenesis. Etoposide, a known topoisomerase II inhibitor induces DNA damage resulting in cell cycle arrest. We developed a novel Etoposide analogue, Quinazolino-4β-amidopodophyllotoxin (C-10) that show better efficacy in regulating cell proliferation and angiogenesis.

Související obsah

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

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