04-282
Anti-phospho-EGFR (Thr654) Antibody, clone 3F2
clone 3F2, Upstate®, from mouse
Synonyma:
ERBB, ERBB1
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O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
3F2, monoclonal
Species reactivity:
mouse, human, canine, rat
Application:
ELISA, IP, WB
Citations:
6
Technický servis
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mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F2, monoclonal
species reactivity
mouse, human, canine, rat
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr654)
Gene Information
dog ... Egfr(404306)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
Application
Detect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
Biochem/physiol Actions
Recognizes phosphorylated EGFR on Threonine 654
Physical form
100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Format: Purified
Analysis Note
Control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Routinely evaluated by immunoblot.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane
Hunter, T, et al
Nature, 311, 480-483 null
Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
Aifa, Sami, et al
Biochemical and biophysical research communications, 347, 381-387 (2006)
Elena Odintsova et al.
The Journal of biological chemistry, 288(36), 26323-26334 (2013-07-31)
Ligand-induced ubiquitylation of EGF receptor (EGFR) is an important regulatory mechanism that controls endocytic trafficking of the receptor and its signaling potential. Here we report that tetraspanin CD82/KAI1 specifically suppresses ubiquitylation of EGFR after stimulation with heparin-binding EGF or amphiregulin