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04-281

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

clone 5F10, Upstate®, from mouse

Synonyma:

ERBB, ERBB1, epidermal growth factor receptor

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O této položce

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
5F10, monoclonal
Species reactivity:
human
Application:
WB
Citations:
7
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biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F10, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005228.3, NM_201282.1, NM_201283.1, NM_201284.1

UniProt accession no.

P00533

shipped in

wet ice

target post-translational modification

phosphorylation (pThr669)

Gene Information

human ... EGFR(1956)

General description

180 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.

Application

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Recognizes EGFR when phosphorylated at Threonine 669.

Physical form

100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

Preparation Note

6 months at -20°C from date of shipment

Analysis Note

Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Skladovací třída

10 - Combustible liquids

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Navštívit knihovnu dokumentů

G J Heisermann et al.
The Journal of biological chemistry, 263(26), 13152-13158 (1988-09-15)
The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
The Journal of Biological Chemistry, 265, 12820-12827 (1990)
Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25, 7381-7390 (2006)
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