If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
753610
Gold nanoparticles
20 nm diameter, OD 1, suspension in 0.1 mM PBS, reactant free
Synonyma:
Au NP, Gold colloid
Vybrat velikost
| Vám/SKU | Dostupnost | Cena |
|---|
O této položce
Quality Segment
form
nanoparticles, suspension
concentration
~6.54E+11 particles/mL
OD
1
diameter
20 nm
λmax
518-522 nm
PDI
<0.2
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
General description
Application
Legal Information
1 of 1
Tato položka | |||
|---|---|---|---|
| description 20 nm diameter, OD 1, suspension in 0.1 mM PBS, reactant free | description 10 nm diameter, OD 1, suspension in citrate buffer | description 40 nm diameter, OD 1, suspension in 0.1 mM PBS, reactant free | description 5 nm diameter, OD 1, suspension in citrate buffer |
| form suspension, nanoparticles | form nanoparticles, suspension | form suspension, nanoparticles | form suspension, nanoparticles |
| diameter 20 nm | diameter 10 nm | diameter 40 nm | diameter 5 nm |
| concentration ~6.54E+11 particles/mL | concentration ~6.0E+12 particles/mL | concentration ~7.2E+10 particles/mL | concentration ~5.5E+13 particles/mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| PDI <0.2 | PDI <0.2 | PDI - | PDI <0.2 |
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Skladovací třída
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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How can I determine the shelf life / expiration / retest date of this product?
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que concentración de partículas tiene
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As listed in the 'PROPERTIES' section, the concentration is ~6.54E+11 particles/mL.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If i want to concentrate or isolate gold mannan particles and remove any unlabeled mannan what filtration or centrifugation conditions should be used.
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This product is comprised of stabilized gold nanoparticles in 0.1 mM PBS. There is no mannan present. The material may be concentrated by centrifugation. Note that aggregation may become an issue if the particles are removed from the stabilizing solution.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Why does my gold nanoparticle solution turn violet when I add salt containing buffer?
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Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no (or small) amounts of electrolytes such as NaCl is present, this energy barrier is too strong for interaction to occur between particles. However, upon addition of NaCl this energy barrier is reduced allowing the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called surface-plasmon coupling which changes the adsorption maximum of light to a higher wavelength resulting in a change in color of the solution.
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Do I need to wash my gold nanoparticles?
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For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing the best way to do so is by either centrifugation or filtration.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
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To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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My gold nanoparticles settle out of solution upon storage. Is this normal?
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Settling of gold nanoparticles on the bottom of the storage flask is completely normal and is especially common for larger sized particles, which settles at greater speed. Settling does not affect the performance of the particles. Prior to use, simply swirl the solution to properly disperse your gold nanoparticles and obtain a homogenous solution.
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How do you attach gold nanoparticles to oligos?
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To attach gold nanoparticles to oligos please view the information in the attached reference - Bioconjug Chem. 2011 Apr 20;22(4):794-807. Epub 2011 Mar 28.
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