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recombinant
expressed in E. coli
Quality Level
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
selection
ampicillin
blasticidin
shipped in
dry ice
storage temp.
−20°C
General description
This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.
Application
Functional Genomics/Screening /Target Validation/Genome Editing
Components
1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin Lenti
Linkage
Click here to order gRNA plasmid and virus.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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