MABT409
Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody
clone AIIB2, 1 mg/mL, from rat
Synonym(s):
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit beta, CD29
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AIIB2, monoclonal
species reactivity
rhesus macaque, horse, human, pig, canine, bovine, sheep
concentration
1 mg/mL
technique(s)
activity assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
General description
Integrin beta-1, also known as Fibronectin receptor subunit beta (FNRB), and Glycoprotein IIa (GPIIA), is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.
Immunogen
Whole cells corresponding to human Integrin Beta1, clone AIIB2 (Azide Free).
Application
Activity Assay Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in this functionally blocking antibody abrogated AGS cell attachment to fibronectin while causing only a slight inhibition of cell attachment to vitronectin (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in floating villi tissue (Damsky, C. H., et al. (1992). Journal of Clinical Investigation. 89:210-222).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in CTB aggregates that have invaded matrigel in vitro (Damsky, C. H., et al. (1994). Development. 120:3657-3666).
Immunocytochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in AGS cells (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin Beta1, clone AIIB2 (Azide Free) in NP-40 cell lysate (Werb, Z., et al. (1989). JCB. 109:877-889).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in floating villi tissue (Damsky, C. H., et al. (1992). Journal of Clinical Investigation. 89:210-222).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in CTB aggregates that have invaded matrigel in vitro (Damsky, C. H., et al. (1994). Development. 120:3657-3666).
Immunocytochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in AGS cells (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin Beta1, clone AIIB2 (Azide Free) in NP-40 cell lysate (Werb, Z., et al. (1989). JCB. 109:877-889).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.
Quality
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin Beta1, clone AIIB2 (Azide Free) in 10 µg of HUVEC cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin Beta1, clone AIIB2 (Azide Free) in 10 µg of HUVEC cell lysate.
Target description
~110 kDa observed. The calculated molecular weight of this protein is 88 kDa, but can be observed at ~110 kDa due to high glycosylation.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Qing Zhao et al.
PLoS pathogens, 14(10), e1007359-e1007359 (2018-10-27)
Translocation of the Helicobacter pylori (Hp) cytotoxin-associated gene A (CagA) effector protein via the cag-Type IV Secretion System (cag-T4SS) into host cells is a hallmark of infection with Hp and a major risk factor for severe gastric diseases, including gastric
Jinhua Zhang et al.
Scientific reports, 7(1), 12099-12099 (2017-09-25)
S100A4 has been implicated in cancer and several inflammatory diseases, but its role in inflammatory bowel disease has not been well investigated. Here, upon infection with Citrobacter rodentium, a model for enteropathogenic Escherichia coli infection in humans, induced the infiltration
Tsunaki Hongu et al.
Nature cancer, 3(4), 486-504 (2022-04-27)
Disseminated cancer cells frequently lodge near vasculature in secondary organs. However, our understanding of the cellular crosstalk invoked at perivascular sites is still rudimentary. Here, we identify intercellular machinery governing formation of a pro-metastatic vascular niche during breast cancer colonization
Charles Kumar Thodeti et al.
FEBS letters, 579(25), 5589-5595 (2005-10-11)
ADAM12, adisintegrin and metalloprotease, has been demonstrated to be upregulated in human malignant tumors and to accelerate the malignant phenotype in a mouse model for breast cancer. ADAM12 is a substrate for beta1 integrins and may affect tumor and stromal
Anh Duy Do et al.
Frontiers in immunology, 12, 796177-796177 (2022-02-01)
Helicobacter pylori is a Gram-negative pathogen that can increase the risk of stomach cancer in infected patients. H. pylori exploits lipid rafts to infect host cells. Infection triggers clustering of Lewis x antigen (Lex) and integrins in lipid rafts to
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