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Key Documents

07-250

Sigma-Aldrich

Anti-PP2A Antibody, A subunit

Upstate®, from rabbit

Synonym(s):

Anti-MRD36, Anti-PP2AA, Anti-PP2AAALPHA, Anti-PR65A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, Xenopus, pig, rat

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002716

UniProt accession no.

P30153

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PPP2R1A(5518)

Specificity

PP2A A subunit, α isoform

Immunogen

KLH-conjugated, synthetic peptide of 13 amino acids (MAAADGDDSLY-Ahx-C), containing amino acids 1-11 of human PP2A, A subunit. Ahx is aminohexanoic acid. The immunizing sequence is conserved in human, mouse, rat, porcine and Xenopus.

Application

Anti-PP2A Antibody, A subunit detects level of PP2A & has been published & validated for use in IP & WB.
Research Sub Category
Synapse & Synaptic Biology

Neurodegenerative Diseases

Quality

Routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells.

Target description

63-65kDa

Physical form

100mM triethylamine, 100mM sodium phosphate, 0.02% sodium azide before the addition of glycerol to 30%.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of the adenovirus E4orf4 protein binding site on the B55? and Cdc55 regulatory subunits of PP2A: Implications for PP2A function, tumor cell killing and viral replication.
Mui, MZ; Kucharski, M; Miron, MJ; Hur, WS; Berghuis, AM; Blanchette, P; Branton, PE
PLoS Pathogens null
G A Calin et al.
Oncogene, 19(9), 1191-1195 (2000-03-14)
The phosphatase 2A (PP2A) is one of the major cellular serine-threonine phosphatases. It was recently shown that the gene encoding for the beta isoform of its subunit A, PPP2R1B, is altered in human lung and colorectal carcinomas, suggesting a role
Sorcha Finnegan et al.
The European journal of neuroscience, 32(3), 322-334 (2010-07-20)
Cell survival signalling involving the PI3K/Akt survival pathway can be negatively regulated by several phosphatases including PP2A. When retinal-derived 661W cells were subjected to trophic factor deprivation this initiated a survival response through inhibition of the activity of PP2A and
The B55? regulatory subunit of protein phosphatase 2A mediates fibroblast growth factor-induced p107 dephosphorylation and growth arrest in chondrocytes.
Kolupaeva, V; Daempfling, L; Basilico, C
Molecular and cellular biology null
Mechanisms of the scaffold subunit in facilitating protein phosphatase 2A methylation.
Stanevich, V; Zheng, A; Guo, F; Jiang, L; Wlodarchak, N; Xing, Y
Testing null
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