Astec CHIROBIOTIC™ TAG columns showing decreased resolution sometimes can be regenerated by passing several column volumes of a 50/50 solution of acetonitrile and 50 mM ammonium acetate (NH4OAc) aqueous buffer. This is followed by passing several column volumes of pure HPLC grade water, and then by passing several column volumes of acetonitrile or methanol, all at a flow rate of 0.5 mL/min. Acetonitrile or methanol may be used for final displacement and storage. For long term storage (>24 hours) isopropanol or methanol is recommended.
14024AST
Astec® CHIROBIOTIC® TAG Chiral (5 μm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
About This Item
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Nombre del producto
Astec® Chirobiotic® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L63
description
HPLC column
product line
Astec®
packaging
pkg of 1 ea
manufacturer/tradename
Astec®
parameter
0-45 °C temperature
241 bar pressure (3500 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
High-purity silica gel particle platform
fully porous particle
matrix active group
teicoplanin aglycone phase
particle size
5 μm
pore size
100 Å
operating pH range
3.0-6.8
separation technique
chiral
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General description
- Bonded phase: Teicoplanin aglycone
- Operating pH range: 3.0 - 6.8
- Particle diameter: 5, 10 or 16 μm
- Pore size: 100 Å
Application
- High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.: This study emphasizes the effectiveness of the Astec® CHIROBIOTIC® TAG Chiral HPLC Column in the enantioseparation of complex cyclic amino acids. The methodology detailed provides profound insights into the chirality aspects of biochemical molecules, which is crucial for the synthesis of novel pharmaceutical agents and the study of biochemical pathways. This research supports analytical chemists in academia focused on developing chiral analytical methods and advancing chemical synthesis (Berkecz et al., 2009).
Legal Information
Application
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Chromatograms
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which is suitable solvent mixture for the cleaning of Astec chirobitic TAG columns (Cat. No. 14024AST)? What is re-generation procedure for this column?
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