ZRB1673
Anti-p-IKKα/β-Ser176/180 Antibody, clone 2G2 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
About This Item
Productos recomendados
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G2, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 84.64 kDa
observed mol wt ~45 kDa
purified by
using Protein A
species reactivity
human
species reactivity (predicted by homology)
canine, bovine, mouse, porcine, rat, monkey, feline
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer176/pSer180)
Gene Information
human ... CHUK(1147)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in a construct containing GST-tagged recombinant IKK /β protein fragment phosphorylated on Ser176/180.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a construct containing GST-tagged recombinant IKK /β protein fragment phosphorylated on Ser176/180, but did not detect in non-phosphorylated construct. (Phospho- IKK /β construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine).
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-IKK /β-Ser176/180 in human colon cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Phospho-IKK /β- Ser176/180 peptide with a KD of 3.2 x 10-7 in an affinity binding assay.
Peptide Inhibition Assay: Target band detection in a construct containing GST-tagged recombinant IKKa protein fragment phosphorylated on Ser 176/180 was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
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Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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