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Y1771

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

Sinónimos:

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements

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About This Item

MDL number:
MFCD01095679
UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

200

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

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Application

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

  • the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
  • Yarrowia lipolytica PO1f strain positive transformants
  • CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

related product

Referencia del producto
Descripción
Precios

G7021

D-(+)-Glucosa, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

Agar, for molecular biology

Y1501

Yeast Synthetic Drop-out Medium Supplements, without uracil

Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y0626

Base de nitrógeno de levadura sin aminoácidos, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2021

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine

Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Paul Swietalski et al.
Yeast (Chichester, England), 39(3), 230-240 (2021-10-15)
Orotic acid (OA) is an intermediate of the pyrimidine biosynthesis with high industrial relevance due to its use as precursor for production of biochemical pyrimidines or its use as carrier molecule in drug formulations. It can be produced by fermentation
Emil D Jensen et al.
Bio-protocol, 12(5), e4346-e4346 (2022-05-21)
Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants with improved traits from vast mutated sequence spaces.
Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

Protocolos

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