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Merck

T8778

Sigma-Aldrich

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sinónimos:

α,α-Trehalose glucohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

buffered aqueous glycerol solution

Quality Level

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:
  • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
  • in a study to investigate growth arrest by trehalose-6-phosphate
  • to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton X-100 and 25 mM potassium phosphate, pH 6.5

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Shari Dhaene et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1964-1989 (2020-11-10)
Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose
Berrak Gülçin Balaban et al.
Microorganisms, 8(1) (2019-12-28)
Iron plays an essential role in all organisms and is involved in the structure of many biomolecules. It also regulates the Fenton reaction where highly reactive hydroxyl radicals occur. Iron is also important for microbial biodiversity, health and nutrition. Excessive
Yu Sasano et al.
Journal of bioscience and bioengineering, 113(5), 592-595 (2012-01-28)
Freeze tolerance is a necessary characteristic for industrial baker's yeast because frozen-dough baking is one of the key technologies for supplying oven-fresh bakery products to consumers. Both proline and trehalose are known to function as cryoprotectants in yeast cells. In
Jiangnan Luo et al.
PloS one, 9(6), e99732-e99732 (2014-06-14)
A set of 14 insulin-producing cells (IPCs) in the Drosophila brain produces three insulin-like peptides (DILP2, 3 and 5). Activity in IPCs and release of DILPs is nutrient dependent and controlled by multiple factors such as fat body-derived proteins, neurotransmitters

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