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Merck

T7408

Sigma-Aldrich

Triethylammonium bicarbonate buffer

1.0 M, pH 8.5±0.1

Sinónimos:

Triethylammonium hydrogen carbonate buffer

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About This Item

Número de CAS:
Beilstein/REAXYS Number:
3624751
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
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form

liquid

Quality Level

storage condition

(Tightly closed)

concentration

1.0 M

color

colorless

pH

8.5±0.1

density

1.02 g/mL at 25 °C (lit.)

suitability

suitable for LC-MS
suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
microbiology
sample preparation

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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Application

Tampón para uso en cromatografía de intercambio iónico y electroforesis.
Triethylammonium bicarbonate buffer is used in ion-exchange chromatography and electrophoresis.
It has also been used:
  • in trypsin digestion[1]
  • as a component of lysis buffer[2]
  • to adjust the pH of buffer for protein quantification[2]

Biochem/physiol Actions

Triethylammonium bicarbonate buffer is useful in oligonucleotides purification for electrospray mass spectrometry analysis.[3] It is also utilized in trypsin digestion buffers.[4]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificados de análisis (COA)

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Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling.
Alonso-Orgaz S, et al.
Methods in Molecular Biology, 109-117 (2013)
Identification of Rosmarinic Acid-Adducted Sites in Meat Proteins in a Gel Model under Oxidative Stress by Triple TOF MS/MS.
Tang CB, et al.
Journal of Agricultural and Food Chemistry, 64(33), 6466-6476 (2016)
Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
Hao P, et al.
Journal of Proteome Research, 14(2), 1308-1314 (2015)
Dwayne A Elias et al.
Nucleic acids research, 37(9), 2926-2939 (2009-03-19)
Hypothetical (HyP) and conserved HyP genes account for >30% of sequenced bacterial genomes. For the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough, 347 of the 3634 genes were annotated as conserved HyP (9.5%) along with 887 HyP genes (24.4%). Given the large
Stefani N Thomas et al.
iScience, 23(6), 101079-101079 (2020-06-14)
The National Cancer Institute (NCI) Clinical Proteomic Tumor Analysis Consortium (CPTAC) established a harmonized method for large-scale clinical proteomic studies. SWATH-MS, an instance of data-independent acquisition (DIA) proteomic methods, is an alternate proteomic approach. In this study, we used SWATH-MS

Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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