T6791

Base Trizma^®

BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

• Sinónimos: 2-Amino-2-(hidroximetil)-1,3-propanodiol, Base tris, THAM, Tris(hidroximetil)-aminometano, Trometamol
• Fórmula lineal: NH₂C(CH₂OH)₃
• Número de CAS: 77-86-1
• Peso molecular: 121.14
• Beilstein/REAXYS Number: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 24900405
• NACRES: NA.25

Propiedades

• description: aminopeptidase substrate
• Quality Level: 400
• product line: BioXtra
• assay: ≥99.9% (titration)
• form: crystalline
• storage condition: dry at room temperature
• impurities: ≤0.005% Insoluble matter
• ign. residue (900°C): ≤0.01% (as SO₄)
• loss: ≤0.5% loss on drying, 110°C
• color: white
• pH: 10.5-12.0 (1 M in H₂O)
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: H₂O: 1 M, colorless; water: 678 g/L at 20 °C
• anion traces: chloride (Cl^-): ≤0.005%; sulfate (SO₄^2-): ≤0.005%
• cation traces: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• SMILES string: NC(CO)(CO)CO
• absorption: ≤0.020 at 280 in H₂O at 1 M; ≤0.025 at 260 in H₂O at 1 M
• application(s): diagnostic assay manufacturing; life science and biopharma; sample preparation
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Descripción

General description

Tris base, also referred as THAM or Trizma^® base, plays a vital role in various research applications as a biological buffer. It is commonly employed in buffer formulations like TAE and TBE buffers, thanks to its pKa of 8.06, which makes it suitable for maintaining pH within the physiological range (pH 7.0-9.0) for most living organisms. However, it is important to exercise caution when studying proteins, as Tris may interfere with the activity of certain enzymes. This versatile compound is indispensable in laboratories, where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Moreover, Tris base finds applications in biochemistry and molecular biology labs aiding in cell membrane permeability studies and buffer preparation.

Application

Tris buffer has been used to stabilize the pH, facilitating the measurement of cytotoxicity using the sulforhodamine B (SRB) colorimetric cytotoxicity assay in cancer research. Additionally, it was utilized in this study to regulate the pH of the incubation medium to assess the glucose secretion as a response mediated by glucocorticoids in metabolic pathways.

Features and Benefits

• Suitable for Cell Membrane permeability studies and for buffer preparation in Biochemical and Molecular Biology Research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested for the presence of Anionic and Cationic traces

Other Notes

Los valores de pH de todas las disoluciones tampón son dependientes de la temperatura y la concentración. Para los tampones Tris, el pH aumenta alrededor de 0,03 unidades por °C de disminución de la temperatura, y disminuye 0,03-0,05 unidades al ser diluido por 10.
Para aplicaciones precisas, debe utilizarse un peachímetro cuidadosamente calibrado con un electrodo de vidrio/calomelano combinados.

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Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves