Saltar al contenido
Merck

SAB4200080

Sigma-Aldrich

Anti-Vinculin Antibody

mouse monoclonal, V284

Sinónimos:

Anti-CMD1W, Anti-MVCL, Anti-VCL

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

En este momento no podemos mostrarle ni los precios ni la disponibilidad

product name

Anti-Vinculin antibody, Mouse monoclonal, clone V284, purified from hybridoma cell culture

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

V284, monoclonal

form

buffered aqueous solution

mol wt

antigen ~117 kDa

species reactivity

rabbit, chicken, human, rat, monkey, mouse, canine

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
C9979SAB4700789SAB4700919
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

1B2, monoclonal

clone

AS32, monoclonal

clone

MMA, monoclonal

clone

R1.302, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... CD151(977)

Gene Information

human ... CTLA4(1493)

Gene Information

human ... FUT4(2526)

Gene Information

human ... NECTIN1(5818)

General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma V284 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified human platelet vinculin. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), neuromuscular junctions, myotendinous junctions (skeletal muscle) and the membrane-associated dense bodies of smooth muscle.
Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

purified human platelet vinculin.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used:
  • to probe blots in immunoblotting
  • immunochemical staining
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Optional

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Necesita un COA de muestra?

Esto es un Certificado de análisis (COA) de muestra y es posible que no represente un lote de fabricación reciente para este producto específico.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Paolo Benatti et al.
Oncotarget, 7(2), 1633-1650 (2015-12-10)
The trimeric transcription factor NF-Y binds to the CCAAT box, an element enriched in promoters of genes overexpressed in tumors. Previous studies on the NF-Y regulome identified the general term metabolism as significantly enriched. We dissect here in detail the
Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes
Geiger B, et al.
Proceedings of the National Academy of Sciences of the USA, 77(7), 4127-4131 (1980)
Gerald S Braun et al.
Journal of the American Society of Nephrology : JASN, 27(1), 132-142 (2015-06-05)
The role of IL-6 signaling in renal diseases remains controversial, with data describing both anti-inflammatory and proinflammatory effects. IL-6 can act via classic signaling, engaging its two membrane receptors gp130 and IL-6 receptor (IL-6R). Alternatively, IL-6 trans-signaling requires soluble IL-6R
The mechanical and biological properties of polycarbonate-modified F127 hydrogels after incorporating active pendent double-bonds
Wang T, et al.
Polymer, 57, 21-28 (2015)
Sex-specific association of rs4746172 of VCL gene with hypertension in two Han populations from Southern China.
Yu Q
Scientific Reports (2015)

Artículos

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico