S2014
Staphylococcus aureus
buffered aqueous suspension, Wood 46 strain
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.81
Productos recomendados
origen biológico
Staphylococcus aureus
Nivel de calidad
esterilidad
Not processed or packaged aseptically
Formulario
buffered aqueous suspension
composición
Cell suspension, ~10% wet weight/volume
temp. de almacenamiento
2-8°C
Descripción general
Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.
Aplicación
(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
- to mimic infection and induce fever in Pekin duck
- to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
- in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt
Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.
Acciones bioquímicas o fisiológicas
Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.
Forma física
Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide
Nota de preparación
Produced in pure culture.
Nota de análisis
This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Los clientes también vieron
M Marais et al.
Poultry science, 90(6), 1234-1238 (2011-05-21)
Poultry, like mammals and other birds, develop fever when exposed to compounds from gram-negative bacteria. Mammals also develop fever when exposed to the constituents of viruses or gram-positive bacteria, and the fevers stimulated by these different pathogenic classes have discrete
S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were
Synthetic modification of gedunin and comparative antibacterial activity of gedunin and 7-deacetoxy-7alpha-hydroxygedunin potassium salt
Okhale SE, et al.
African Journal of Pure and Applied Chemistry, 6(14), 183-189 (2012)
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
Manasi Balachandran et al.
PloS one, 12(8), e0183913-e0183913 (2017-09-01)
Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control
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