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Merck

S0146

Sigma-Aldrich

Schneider′s Insect Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Sinónimos:

Schneider’s Drosophila Medium

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75
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Nombre del producto

Schneider′s Insect Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other dipteran cell lines (Originally developed for the culture of Drosophila cells)

components

NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines.
Schneider′s insect medium has been used:
  • to culture Drosophila neuronal cell line (BG3-c2 cells)[1]
  • to cultivate subcultures of Leishmania major for promastigote growth inhibition assay[2]
  • to seed purified ookinetes with Drosophila melanogaster S2 cells to assess the effect of the avermectin compound on cell development and to promote oocyte development[3]

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Anna Franz et al.
The Journal of cell biology, 203(5), 785-799 (2013-12-04)
CP110 is a conserved centriole protein implicated in the regulation of cell division, centriole duplication, and centriole length and in the suppression of ciliogenesis. Surprisingly, we report that mutant flies lacking CP110 (CP110Δ) were viable and fertile and had no
Eva Wegel et al.
Scientific reports, 6, 27290-27290 (2016-06-07)
Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution
Amanda R Brock et al.
Genetics, 206(3), 1505-1520 (2017-05-18)
Regeneration is a complex process that requires an organism to recognize and repair tissue damage, as well as grow and pattern new tissue. Here, we describe a genetic screen to identify novel regulators of regeneration. We ablated the
Amit Sinha et al.
Genome biology and evolution, 11(3), 706-720 (2019-02-05)
Wolbachia, an alpha-proteobacterium closely related to Rickettsia, is a maternally transmitted, intracellular symbiont of arthropods and nematodes. Aedes albopictus mosquitoes are naturally infected with Wolbachia strains wAlbA and wAlbB. Cell line Aa23 established from Ae. albopictus embryos retains only wAlbB
Poonam Aggarwal et al.
Genetics, 207(1), 197-213 (2017-07-12)
One of the pertinent issues associated with cellular plasticity is to understand how the delicate balance between the determined state of cells and the extent to which they can transdetermine is maintained. Employing the well-established model of generating ectopic eyes

Questions

  1. Is it possible to freeze this medium ? I'll need to use only a few millilitres for each dissections of fly brains so it'll be great if I could freeze it in smaller containers to extend the shelf life period.

    1 answer
    1. This medium has not been evaluated for freezer storage. The thawing process may cause precipitation of some salts. The end user may determine the suitability experimentally by freezing a small test volume. It is advisable to thaw the product slowly to minimize the chance of precipitation. Thaw the sample slowly, overnight in a refrigerator., if possible It will very likely be suitable if the resulting thawed medium is clear, colorless, and free of particulate matter. Do not add serum or supplements prior to freezing the test sample.

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