P1291
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Antiperoxidase, Pap Antibody, Peroxidase Anti Peroxidase, Peroxidase Anti Peroxidase - Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit, PAP
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About This Item
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
form
buffered aqueous solution
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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Specificity
By immunoelectrophoresis (IEP), a single arc of precipitation is observed when the product (prior to the addition of BSA) is electrophoresed and then allowed to diffuse against anti-rabbit whole serum or anti-rabbit IgG. No reaction is observed against free horseradish peroxidase.
Application
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit has been used in:
- immunoblotting
- to detect the expression of TAP-tagged proteins
- immunoprecipitation
- immunohistochemical staining
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit was used in immunoblotting of yeast whole cell extracts.
Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Sigma Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Biochem/physiol Actions
Peroxidase:anti-peroxidase (PAP) immune complex is inexpensive immunoglobulin ligand. It is widely used for the detection of fragment crystallisable (Fc) receptor binding and fragment antigen binding (Fab) regions.
The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing or storage in "frost-free" freezers, is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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