Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

MAK136

Sigma-Aldrich

Monoamine Oxidase Activity Assay Kit

Monoamine Oxidase Activity Assay Kit

sufficient for 100 fluorometric tests

Sinónimos:

MAO Assay Kit

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... MAOA(4128) , MAOB(4129)
mouse ... MAOA(17161) , MAOB(109731)
rat ... MAOA(29253) , MAOB(25750)

General description

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.

Application

Monoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A)[1][2], MAO-B (monoamine oxidase B)[1] and ALDH (aldehyde dehydrogenase) activities.[2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.

Principle

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex = 530/λem = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 μmole of H2O2 per minute under the assay conditions.

Storage Class

10 - Combustible liquids

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
136CE01A02
136CD07A06
136CE02A08
136CD05A15
136CC07A25

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Umut Salgin-Goksen et al.
Journal of medicinal chemistry, 64(4), 1989-2009 (2021-02-04)
Thirty compounds having 1-[2-(5-substituted-2-benzoxazolinone-3-yl) acetyl]-3,5-disubstitutedphenyl-2-pyrazoline structure and nine compounds having N'-(1,3-disubstitutedphenylallylidene)-2-(5-substituted-2-benzoxazolinone-3-yl)acetohydrazide skeleton were synthesized and evaluated as monoamine oxidase (MAO) inhibitors. All of the compounds exhibited selective MAO-A inhibitor activity in the nanomolar or low micromolar range. The results of
Jon-Ruben van Rhijn et al.
Neurobiology of disease, 163, 105587-105587 (2021-12-20)
Monoamine neurotransmitter abundance affects motor control, emotion, and cognitive function and is regulated by monoamine oxidases. Among these, Monoamine oxidase A (MAOA) catalyzes the degradation of dopamine, norepinephrine, and serotonin into their inactive metabolites. Loss-of-function mutations in the X-linked MAOA
Lovedeep Singh et al.
Metabolic brain disease, 36(1), 111-121 (2020-09-02)
Pain and depression are often co-existing pathological states that promote mutual severity resulting in limited efficacy of current treatment strategies. Thus, there is a need to develop an efficacious alternate treatment regimen for pain-depression dyad. Skimmetin and osthole are molecules
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Elevates Stimulus-Evoked Release of Dopamine in Freely-Moving Rats.
Renko J M, et al.
Molecular Neurobiology, 1-14 (2018)
Juho-Matti Renko et al.
Molecular neurobiology, 55(8), 6755-6768 (2018-01-20)
Neurotrophic factors (NTFs) hold potential as disease-modifying therapies for neurodegenerative disorders like Parkinson's disease. Glial cell line-derived neurotrophic factor (GDNF), cerebral dopamine neurotrophic factor (CDNF), and mesencephalic astrocyte-derived neurotrophic factor (MANF) have shown neuroprotective and restorative effects on nigral dopaminergic
Ver todos los artículos relacionados
1 Review This action will navigate to reviews.

Questions

1–2 of 2 Questions  

  1. What is the concentration of the p-tyramine stock and the HRP enzyme that is included in the kit?

    1 answer

    1. The concentration of the p-tyramine included in the kit is 100mM, and the enzyme concentration is 0.02 U/uL.

      Helpful?

  2. Is it possible to determine if this kit would be suitable for the quantitation of MAO-N?

    1 answer

    1. This kit is not tested with MAO-N. It is important to note that this kit was specifically developed with MAO-A and MAO-B in mind, and it is uncertain whether other enzymes, such as MAO-N, would be compatible with the substrate.

      Helpful?

Reviews

Active Filters

  1. New York, NY
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Performed Flawlessly

    This has produced consistent results every single time.

    Helpful?

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico