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Merck

MAK118

Sigma-Aldrich

Invertase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

β-fructofuranosidase Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

aplicaciones

cosmetics
environmental
food and beverages

método de detección

colorimetric
fluorometric

temp. de almacenamiento

−20°C

Descripción general

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

Aplicación

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

Principio

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.

Código de clase de almacenamiento

10 - Combustible liquids


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Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
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