The positive control is glucose 6 phosphate dehydrogenase from Leuconostoc mesenteroides, purified from E. coli.
MAK015
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit
sufficient for 100 colorimetric tests
Sinónimos:
G-6-PD Activity Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
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uso
sufficient for 100 colorimetric tests
método de detección
colorimetric
enfermedades relevantes
cancer; hematological disorder
temp. de almacenamiento
−20°C
Información sobre el gen
human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)
Descripción general
Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP).[1] The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars.[2] The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis.[3] Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.[4]
Aplicación
Idoneidad
Suitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.
Principio
In the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.
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Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Dam. 1 - Skin Corr. 1B
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Living with Glucose-6-Phosphate Dehydrogenase Deficiency
Devi A S, et al.
Journal of nursing science and practice., 6(1) (2016)
Rapid Elevation in CMPF May Act As a Tipping Point in Diabetes Development
Ying L, et al.
Cell Reports, 14(12), 2889-2900 (2016)
Nuclear Heme Oxygenase-1 (HO-1) Modulates Subcellular Distribution and Activation of Nrf2, Impacting Metabolic and Anti-oxidant Defenses.
Chhanda B, et al.
The Journal of Biological Chemistry, jbc-M114 (2014)
Redox Regulation of Cell Survival.
Dunyaporn T, et al.
Antioxidants & Redox Signaling, 10(8), 1343-1374 (2008)
Sandra Meyer et al.
Nutrients, 12(4) (2020-04-08)
The present study tested the hypothesis that the liver lipid-lowering effect of insect meal (IM) is caused by its low methionine concentration. A total of fifty, male obese Zucker rats were randomly assigned to five groups of 10 rats each
Protocolos
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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Is the positive control an enzyme for dehydrogenase?
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