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Merck

M7523

Sigma-Aldrich

Anti-Myosin (Skeletal) antibody produced in rabbit

enhanced validation

whole antiserum

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human or animal skeletal muscle
indirect immunofluorescence: 1:20 using human or animal sletetal muscle

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Myosin is a 480,000 dalton protein containing two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region. Multiple forms of myosin heavy chains exist for each muscle type; skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle.

Specificity

Specifically labels the A bands of human and animal skeletal muscle. Does not stain human smooth muscle tissue.

Immunogen

whole myosin (heavy and light chains) from human skeletal muscle.

Application

Anti-Myosin (Skeletal) antibody produced in rabbit has been used in:
  • immunohistochemistry
  • immunostaining
  • western blotting
  • immunofluorescence
  • immunolocalization
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Quality

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Małgorzata Zimowska et al.
International journal of molecular sciences, 21(9) (2020-05-13)
Skeletal muscle regeneration depends on the satellite cells, which, in response to injury, activate, proliferate, and reconstruct damaged tissue. However, under certain conditions, such as large injuries or myopathies, these cells might not sufficiently support repair. Thus, other cell populations
Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures
Jazedje T, et al.
Journal of Translational Medicine, 7(1), 1-10 (2009)
Tatiana Jazedje et al.
Journal of translational medicine, 7, 46-46 (2009-06-23)
The possibility of using stem cells for regenerative medicine has opened a new field of investigation. The search for sources to obtain multipotent stem cells from discarded tissues or through non-invasive procedures is of great interest. It has been shown
Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood!
Secco M, et al.
Stem Cells, 26(1), 146-150 (2008)
K Vijayan et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1017-1023 (1998-09-08)
Sarcomere lesions were previously observed with reloading of rat adductor longus muscles after spaceflight and hindlimb unloading (HU). Spaceflown rats displayed more lesioned fibers in the "slow-fiber" region, suggesting a damage-susceptible fiber type. Unloading induces fast myosin expression in some

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