L7395
Lipoxidase from Glycine max (soybean)
Type I-B, lyophilized powder, ≥50,000 units/mg solid
Sinónimos:
15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
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About This Item
Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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Type I-B
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥50,000 units/mg solid
mol peso
94 kDa
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
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Descripción general
Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.
Aplicación
Lipoxidase from Glycine max (soybean) has been used:
- to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
- as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
- as a positive control in lipoxygenase inhibitory activity assay
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Acciones bioquímicas o fisiológicas
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Definición de unidad
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Forma física
Contains stabilizer and NaCl
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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