I9889

Anti-Human IgA (α-chain specific) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Número MDL: MFCD00162412
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 300
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• técnicas: : 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum; : 4.0-6.0 mg/mL using Antibody concentration by precipitin Vs Human whey; Ouchterlony double diffusion: 1:32
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

IgA is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa.
Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins.

Immunoglobulin A (IgA) exists in two isotypic forms: IgA1 and IgA2. It is produced as secretory IgA (sIgA) and serum IgA. sIgA exists in a monomeric form with two heavy and two light chains. Serum IgA exists in the dimeric form linked by disulfide bind with an additional J chain. IgA is found in saliva, serum, colostrum and nasal fluid.

Especificidad

The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins.

Inmunógeno

IgA purified from normal human colostrum

Aplicación

Anti-Human IgA (α-chain specific) antibody is suitable for use in:

• enzyme-linked immunosorbent assay (ELISA)
• quantitative precipitin assay (2.5-5 mg/mL)
• immunoelectrophoresis
• Ouchterlony double diffusion (1:16)

Acciones bioquímicas o fisiológicas

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It prevents the commensal microorganisms from invading the intestines.

Immunoglobulin A (IgA) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to leukocytes and enhances microbial destruction. It acts as the first line of defense in antigen clearance. IgA initiates the alternative pathway of the complement system to perform protective functions. It reduces inflammation stimulated by the classical pathway complement activation. IgA prevents proteolysis and inhibits the uptake of bacterial, viral or antigen attachment.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable