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Merck

C3228

Sigma-Aldrich

CelLytic MT Cell Lysis Reagent

For mammalian tissues

Sinónimos:

Cell lysis buffer, Cell lysis reagent

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

Nivel de calidad

Formulario

solution

Aplicación

CelLytic MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Características y beneficios

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Otras notas

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Calidad

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Información legal

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Leticia E Camacho et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(2), R101-R109 (2017-05-12)
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The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
Pasireotide protects mammalian cochlear hair cells from gentamicin ototoxicity by activating the PI3KAkt pathway
Kucharava K, et al.
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Aerobic exercise, but not metformin, prevents reduction of muscular performance by AMPk activation in mice on doxorubicin chemotherapy
de Lima EA, et al.
Journal of Cellular Physiology, 233(12), 96529662-96529662 (2018)

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