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I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

Código UNSPSC:
12352205
NACRES:
NA.75

esterilidad

sterile-filtered

Nivel de calidad

500

Línea del producto

BioReagent

Formulario

liquid

técnicas

cell culture | mammalian: suitable
single cell analysis: suitable

impurezas

endotoxin, tested

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Categorías relacionadas

Aplicación

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Acciones bioquímicas o fisiológicas

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Componentes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Otras notas

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Nota de preparación

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000366647
0000366647
0000359154
0000346703
0000359154

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Christine M Reich et al.
Veterinary research communications, 36(2), 139-148 (2012-03-07)
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
Loraine L Y Chiu et al.
The Laryngoscope, 127(3), E91-E99 (2016-11-20)
Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit
Cyrill Géraud et al.
Hepatology (Baltimore, Md.), 52(1), 313-326 (2010-06-26)
Liver sinusoidal endothelium (LSEC) is a prime example of organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program
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