H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

lyophilized powder, ≥200 units/mg protein

• Sinónimos: (R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH
• Número de CAS: 9028-38-0
• Comisión internacional de enzimas: 1.1.1.30 (BRENDA, IUBMB)
• MDL number: MFCD00130633
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: bacterial (Pseudomonas lemoignei)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥200 units/mg protein
• purified by: chromatography
• foreign activity: lactic dehydrogenase ≤0.05%; malic dehydrogenase ≤0.1%
• storage temp.: −20°C

Descripción

General description

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

Application

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Physical form

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)