GE28-9180-08
PD Miditrap G-25
Cytiva 28-9180-08
Sinónimos:
G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap
About This Item
Productos recomendados
Materiales
polypropylene and polyethylene resin
resin (Sephadex G-25)
esterilidad
non-sterile
Características
wetted part: no
envase
pkg of 50 ea (columns)
fabricante / nombre comercial
Cytiva 28-9180-08
condiciones de almacenamiento
dry media
lecho tamaño
13 mm × 26 mm
lecho volumen
3.5 mL
columna D.I.
13 mm
volumen de trabajo
0.5—1 mL
Matriz
cross-linked dextran
tamaño de partícula
>50 μm (dry)
85-260 μm
tamaño de poro
5000 Da MWCO
cleaning
2-13
Intervalo de trabajo
2-13
idoneidad
suitable for bioprocess medium
Categorías relacionadas
Descripción general
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
Aplicación
Características y beneficios
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
Almacenamiento y estabilidad
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
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Certificados de análisis (COA)
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Artículos
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Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protocolos
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
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