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GE28-9180-08

PD Miditrap G-25

Cytiva 28-9180-08

Sinónimos:

G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap

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About This Item

Código UNSPSC:
41115709
NACRES:
NA.56

Características

wetted part: no

envase

pkg of 50 ea (columns)

fabricante / nombre comercial

Cytiva 28-9180-08

condiciones de almacenamiento

dry media

lecho tamaño

13 mm × 26 mm

lecho volumen

3.5 mL

columna D.I.

13 mm

Matriz

cross-linked dextran

tamaño de partícula

>50 μm (dry)
85-260 μm

cleaning

2-13

Intervalo de trabajo

2-13

idoneidad

suitable for bioprocess medium

Descripción general

PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.

PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.

Aplicación

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Características y beneficios

  • Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
  • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
  • High desalting capacity.
  • Flexible protocols with ability to use gravity flow or centrifugation.
  • Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2


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Artículos

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

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