GE28-9042-67
illustra™ Plasmid Midi (25)
Cytiva 28-9042-67, pack of 1 × 25 pieces
About This Item
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packaging
pack of 1 × 25 pieces
manufacturer/tradename
Cytiva 28-9042-67
General description
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
Features and Benefits
- Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
- Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA
Storage and Stability
Analysis Note
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Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
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Artículos
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
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