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GE17-0408-01

HiTrap® Chelating High Performance

Cytiva 17-0408-01, pack of 5 × 1 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

envase

pack of 5 × 1 mL

fabricante / nombre comercial

Cytiva 17-0408-01

Parámetros

<4 mL/min flow rate
42 psi

lecho tamaño

7 mm × 25 mm

lecho volumen

1 mL

columna D.I.

7 mm

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning

2-14

Intervalo de trabajo

3-13

Categorías relacionadas

Descripción general

HiTrap® Chelating HP is prepacked with Chelating Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Aplicación

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System

Características y beneficios

  • For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose High Performance.
  • Purifying Proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC System.
  • Packed with Chelating Sepharose High Performance
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Zhiyong Chen et al.
Kidney international, 97(6), 1150-1163 (2020-02-18)
Epithelial cells lining the proximal tubule of the kidney reabsorb and metabolize most of the filtered low-molecular-weight proteins through receptor-mediated endocytosis and lysosomal processing. Congenital and acquired dysfunctions of the proximal tubule are consistently reflected by the inappropriate loss of

Artículos

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

Ver todo

Protocolos

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

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