G9043
Anti-GRP78/BiP (ET-21) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinónimos:
Anti-78-kDa Glucose-Regulating Protein, Anti-Immunoglobulin Heavy Chain Binding Protein
About This Item
Productos recomendados
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 78 kDa
species reactivity
mouse, chicken, Xenopus, hamster, human, rat
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: 1:1,000 using methanol/acetone fixed cultured mouse fibroblast NIH3T3 cell line
immunohistochemistry: 1:1,000 using human cerebral cortex and human thyroid gland tissue sections
western blot: 1:3,000 using whole cell extract of human epitheloid carcinoma HeLa cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSPA5(3309)
mouse ... Hspa5(14828)
rat ... Hspa5(25617)
Application
- for immunofluorescence staining of frozen heart tissue to examine whether ER stress signaling activation occurs in cardiomyocytes
- for immunohistochemistry on Paraffin embedded sections of human cerebral cortex and human thyroid gland tissue sections.
- to detect Bip by immunoblotting of protein extract from WT AB strain of zebrafish (Danio rerio)
- at a working dilution of 1:1000 to detect GRP78 in extract of prostate cancer cells after androgen deprivation therapy
Biochem/physiol Actions
Physical form
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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