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Merck

G5154

Sigma-Aldrich

Glasgow Minimum Essential Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

BHK medium, GMEM, Glasgow′s MEM

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Reconstitution

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Expression of the transcription factors OCT4, SOX2, KLF4, and cMYC (OSKM) reprograms somatic cells into induced pluripotent stem cells (iPSCs). Reprogramming is a slow and inefficient process, suggesting the presence of safeguarding mechanisms that counteract cell fate conversion. One such

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