ECAS9GFPPR
eSpCas9-GFP Protein
from Streptococcus pyogenes with mutations conferring enhanced specificity, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS
Sinónimos:
eSpCas9-EGFP, eSpCas9-GFP
About This Item
Productos recomendados
recombinante
expressed in E. coli
Ensayo
≥90% (SDS-PAGE)
Formulario
lyophilized powder
envase
pkg of 1 kit (3 components)
gen reportero
GFP
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
An N-terminally fused enhanced green fluorescent protein with an excitation peak at 488 nm and emission peak at 509 nm allows for visualization of transfected RNP complex in addition to utility in flow cytometry applications. The protein also contains three varied nuclear localization sequences postioned for optimal activity.
Aplicación
- Functional Genomics
- Target Validation
- Genome Editing
- Fluorescence Microscopy
- Flow Cytometry
Características y beneficios
- Enhanced specificity compared to wild type Cas9
- Highly active
- Ready-to-inject/transfect
Envase
pkg of 250 μg ( ≥1300 pmol)
Componentes
- one vial of lyophilized eSpCas9-GFP recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
Principio
Newly engineered eSpCas9 (1.1) enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations (K848A/K1003A/R1060A) in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.
Reconstitución
Otras notas
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Información legal
Código de clase de almacenamiento
11 - Combustible Solids
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Certificados de análisis (COA)
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Protocolos
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
Contenido relacionado
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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