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E6511

Sigma-Aldrich

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

BioReagent, suitable for cell culture, 98.5-102.0%

Sinónimos:

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

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About This Item

Fórmula lineal:
(NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O
Número de CAS:
10378-23-1
Peso molecular:
416.20
Beilstein/REAXYS Number:
3861753
EC Number:
200-573-9
MDL number:
MFCD00150026
UNSPSC Code:
12352204
PubChem Substance ID:
24894629
NACRES:
NA.75

product line

BioReagent

Quality Level

200

assay

98.5-102.0%

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 160 mg/mL

shipped in

ambient

storage temp.

room temp

SMILES string

O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4

InChI key

KSYNLCYTMRMCGG-UHFFFAOYSA-J

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General description

Ethylenediaminetetraacetic acid (EDTA) is a chelator of metal ions. It is a substituted diamine, which has antibacterial activity. EDTA removes the undesirable effects of ferric, cupric and manganic ions in bleaching. It prevents cellular division, chlorophyll synthesis and algal biomass production. EDTA is an inhibitor of metalloprotease. It has anticoagulant property.

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:
  • as a chelating agent to isolate human endometrial stem cell/stromal cells (hEnSCs) from menstrual blood
  • as a chelator in animal and testicular cell isolation
  • as a chelator for the culture of neural stem cells

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Modulation of matrix metalloproteinase activity by EDTA prevents posterior capsular opacification
Hazra S, et al.
Molecular Vision, 18(6), 1701-1701 (2012)
Accurate Results in the Clinical Laboratory: A Guide to Error Detection and Correction (2013)
What is the best protocol to cryopreserve immature mouse testicular cell suspensions?
Onofre J, et al.
Reproductive Biomedicine Online, 37(1), 6-17 (2018)
Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
Tavakol S, et al.
Bio-protocol, 8(2) (2018)
Mary L Bath
PloS one, 5(2), e9387-e9387 (2010-03-03)
In vitro fertilization (IVF) of eggs by frozen and thawed C57BL/6J mouse sperm is inhibited by dead sperm and enhanced by preincubation of the sperm in calcium-free medium. In other species, the presence of sperm killed by freezing and thawing
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