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D0550

Sigma-Aldrich

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Sinónimos:

3,5-Dinitro-2-hydroxybenzoic acid, DNS

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About This Item

Fórmula lineal:
(O2N)2C6H2-2-(OH)CO2H
Número de CAS:
609-99-4
Peso molecular:
228.12
Beilstein/REAXYS Number:
2220661
EC Number:
210-204-3
MDL number:
MFCD00007104
UNSPSC Code:
12171500
PubChem Substance ID:
24893243
NACRES:
NA.47

Quality Level

200

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

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Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Gusakov, A.V., et al.
International Journal of Antimicrobial Agents, e283658-e283658 (2011)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)
Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.
Breuil, C. and Saddler, J.N.
Enzyme and Microbial Technology, 7, 327-332 (1985)
Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.
J F Robyt et al.
Analytical biochemistry, 45(2), 510-516 (1972-02-01)
Rammohan Subramanian et al.
Acta biochimica Polonica, 55(2), 391-398 (2008-05-31)
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro
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