NCTC clone 1469

88111403, mouse liver, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: NCTC clone 1469, 88111403
• origen biológico: mouse liver
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C3H/An liver

Descripción línea celular

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Aplicación

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Medio de cultivo

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

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