Saltar al contenido
Merck

CA201

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 96 assays

Quality Level

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CAMP(820)

General description

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Application

cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.

Other Notes

Please refer to the attached Protocolfor details.

Solo componentes del kit

Referencia del producto
Descripción

  • Acetic Anhydride 1 x 1

  • Assay Buffer 2 1 x 30

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP Assay Layout Sheet 1 x 1

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

Ver todo (13)

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

target_organs

Respiratory system

supp_hazards

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Jonathan Mowers et al.
eLife, 2, e01119-e01119 (2013-12-26)
Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1
The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae
Kalivoda EJ, et al.
Applied and Environmental Microbiology, 74(11), 3461-3470 (2008)
Abstracts of the XXIII Congress of the International Society on Thrombosis and Haemostasis with the 57th Annual SSC (Scientific and Standardization Committee) Meeting. July 23-28 2011. Kyoto, Japan.
Journal of thrombosis and haemostasis : JTH, 9 Suppl 2, 1-1055 (2011-08-10)
Monika Schmoll et al.
Eukaryotic cell, 8(3), 410-420 (2009-01-13)
Although the enzymes enabling Hypocrea jecorina (anamorph Trichoderma reesei) to degrade the insoluble substrate cellulose have been investigated in some detail, little is still known about the mechanism by which cellulose signals its presence to the fungus. In order to
Lingyue Yuan et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 40(10), 1465-1471 (2020-10-30)
To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico