C2306
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Anti-Rabbit IgG Cy3, Cy3 Anti-Rabbit IgG
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About This Item
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Productos recomendados
origen biológico
sheep
Nivel de calidad
conjugado
CY3 conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
reactividad de especies
rabbit
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Descripción general
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep binds to all rabbit Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.
The monomeric structure of immunoglobulin G (IgG) consists of two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments).
Aplicación
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment-Cy3 antibody produced in sheep may be used for immunohistochemistry at a dilution of 1:100 and for immunocytochemistry.
Acciones bioquímicas o fisiológicas
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment antibody allows for the visualization of proteins by fluorescent microscopy.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Información legal
Cy is distributed under license from Amersham Biosciences Limited.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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