Saltar al contenido
Merck
Todas las fotos(6)

Key Documents

View All Documentation

C1403

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas sp.

lyophilized powder, ≥200,000 units/g protein

Sinónimos:

Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(6)

About This Item

Número de CAS:
9026-00-0
Comisión internacional de enzimas:
3.1.1.13 (BRENDA, IUBMB)
EC Number:
232-808-6
MDL number:
MFCD00071052
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥200,000 units/g protein

mol wt

~300 kDa

composition

protein, ≥40% biuret

storage temp.

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Application

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.

Physical properties

Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Javier Sánchez-Marco et al.
Biomedicines, 10(3) (2022-03-26)
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed.
Kevin R Shreder et al.
Bioorganic & medicinal chemistry letters, 22(17), 5748-5751 (2012-08-11)
KIAA1363 is a serine hydrolase whose activity has been shown to be positively associated with tumor cell invasiveness. Thus, inhibitors of KIAA1363 represent a novel targeted therapy approach towards cancer. AX11890 ((1-bromo-2-naphthyl) N,N-dimethylcarbamate) was identified as a KIAA1363 inhibitor with
David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Pornpen Srisawasdi et al.
Journal of clinical laboratory analysis, 26(6), 420-430 (2012-11-13)
Accurate determination of cholesterol requires complete hydrolysis of cholesteryl esters and must be very fast for the kinetic cholesterol assay. We investigated the properties of cholesterol esterase derived from Pseudomonas fluorescens, Candida cylindracea, bovine pancreas, and porcine pancreas for cholesterol
Farid Shokry Ataya
Molecules (Basel, Switzerland), 17(9), 10399-10413 (2012-09-01)
Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first
Ver todos los artículos relacionados

Artículos

Cholesterol Esterification

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

Cholesterol Esterification

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

Cholesterol Esterification

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

Cholesterol Esterification

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

Protocolos

Assay Procedure for Cholesterol Esterase

Protocol for Assay Procedure for Cholesterol Esterase

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico