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B6916

Supelco

Reactivo Bradford

Bradford Reagent

for 0.1-1.4 mg/ml protein

Sinónimos:

Ensayo de unión de las proteínas al colorante Coomassie, reactivo colorante proteico

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About This Item

UNSPSC Code:
12161500
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

200

form

solution

storage temp.

2-8°C

General description

El análisis de Bradford consiste en añadir azul brillante de Coomassie G-250 a la disolución proteica. El colorante azul Coomassie se asocia con los aminoácidos básicos y aromáticos, causando de este modo un viraje en la absorbancia durante la determinación de proteínas.[1]

Application

El reactivo de Bradford se ha utilizado para determinar la concentración total de proteínas.[2][3][4]

Features and Benefits

  • El reactivo está listo para usar. No se requiere mezcla ni dilución.
  • La aparición del color es rápida. Bastan 5 minutos de incubación y la muestra puede leerse a 595 nm.
  • Los azúcares reductores y las sustancias reductoras junto con los tioles no interfieren con este reactivo.
  • El reactivo es adecuado para microanálisis (1 - 10 μg/ml) y análisis convencionales (50-1400 μg/ml).
  • Puede utilizarse en análisis de placas de micropocillos.
  • Análisis barato.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Legal Information

Application

Referencia del producto
Descripción
Precios

also commonly purchased with this product

Referencia del producto
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related product

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Descripción
Precios

TP0100

Total Protein Kit, Micro

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000261841
0000261841
SLCN3585
SLCK1933
SLCG1879

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Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
Kapil Dev Singh et al.
PloS one, 11(3), e0149418-e0149418 (2016-03-18)
Complex traits, including common disease-related traits, are affected by many different genes that function in multiple pathways and networks. The apoptosis, MAPK, Notch, and Wnt signalling pathways play important roles in development and disease progression. At the moment we have
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
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Protocolos

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Contenido relacionado

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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Questions

1–10 of 11 Questions  

  1. Talking about the Bradford reagent, I read that: The linear concentration range is 0.1-1.4 mg/mL of protein... does this mean I can prepare these concentrations of protein which will then be diluted when mixed with a certain amount of reagent?

    1 answer

    1. For the Standard 3.1 mL Assay and 96 Well Plate Assay, the protein standards should be prepared in the range of 0.1 - 1.4 mg/mL. This is not the final concentration of the protein after being diluted in the assay.

      For the full protocol, please refer to the document found here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/165/479/b6916bul-ms.pdf

      Helpful?

  2. Hallow, Does Bradford Reagent B6916 have color? I don't see it the SDS

    1 answer

    1. The color of this product can range from Faint Yellow-Brown to Light Brown. The exact result will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’: https://www.sigmaaldrich.com/product/sigma/b6916#product-documentation

      Helpful?

  3. Does this product (B6916) suitable for use with buffers with 1% SDS?

    1 answer

    1. A compatibility chart is listed in the bulletin. This chart indicates that this reagent is compatible with concentrations of SDS up to 0.125%.

      Helpful?

  4. Can i use this reagent to quantify protein range from 0-10 ug? May i dilute this bradford reagent, and if i do, will it affect the accuracy of the reading?

    1 answer

    1. The Bradford method has a lower limit of detection of 20 ug/mL. The Lowry method has a lower limit of 10 ug/mL. Concentration of the sample may be necessary.
      See the links below for additional helpful information:

      Protein Quantitation Methods-
      https://www.sigmaaldrich.com/applications/protein-biology/protein-quantitation

      Amicon Centrifugal Filters-
      https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters

      Centricon Centrifugal Filters-
      https://www.sigmaaldrich.com/substance/centriconplus70centrifugalfilter1234598765

      Helpful?

  5. How long is the  reagant stable or can it separate and require mixing prior to use? 

    1 answer

    1. Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat. When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. At somewhat lower temperatures aggregation is also expected to occur, but at relatively slower rates.

      Please see the product data sheet which describes the solution stability of BSA:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/351/531/a8412pis.pdf

      Helpful?

  6. When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?

    1 answer

    1. he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.

      Helpful?

  7. Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?

    1 answer

    1. A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.

      Helpful?

  8. When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?

    1 answer

    1. The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?

    1 answer

    1. The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.

      Helpful?

1–10 of 11 Questions  

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