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Merck
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A5588

Sigma-Aldrich

Anti-polihistidina monoclonal

clone HIS-1, purified from hybridoma cell culture

Sinónimos:

Monoclonal etiqueta 6xHis, Monoclonal etiqueta His, Monoclonal etiqueta His6, Monoclonal etiqueta de epítopo 6 H, Monoclonal etiqueta de epítopo HHHHHH, Monoclonal etiqueta hexa His, Monoclonal etiqueta poli-His, Monoclonal etiquetado con histidina

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

buffered aqueous glycerol solution

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

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General description

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificity

El anticuerpo reconoce la polihistidina sintética, así como las formas reducidas, nativas o desnaturalizadas, de proteínas etiquetadas con 6 histidinas, expresadas en vectores seleccionados.

Immunogen

Proteína de fusión recombinante etiquetada con polihistidina.

Application

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dobo,J et al.
Applied and Environmental Microbiology, 6(3), 891-899 (2010)
Rozbeh Jafari et al.
Microbial cell factories, 10, 34-34 (2011-05-18)
Optimization of conditions during recombinant protein production for improved yield is a major goal for protein scientists. Typically this is achieved by changing single crucial factor settings one at a time while other factors are kept fixed through trial-and-error experimentation.
Pooja Bhardwaj et al.
Antimicrobial agents and chemotherapy, 60(4), 2209-2221 (2016-01-27)
Chlorhexidine is a bisbiguanide antiseptic used for infection control. Vancomycin-resistantE. faecium(VREfm) is among the leading causes of hospital-acquired infections. VREfm may be exposed to chlorhexidine at supra- and subinhibitory concentrations as a result of chlorhexidine bathing and chlorhexidine-impregnated central venous
Sarah E Partovi et al.
The Journal of biological chemistry, 293(14), 5236-5246 (2018-02-08)
For nearly 30 years, coenzyme M (CoM) was assumed to be present solely in methanogenic archaea. In the late 1990s, CoM was reported to play a role in bacterial propene metabolism, but no biosynthetic pathway for CoM has yet been
Richard Glatz et al.
The Journal of biological chemistry, 278(22), 19743-19750 (2003-03-20)
Polydnaviruses are essential for the survival of many Ichneumonoid endoparasitoids, providing active immune suppression of the host in which parasitoid larvae develop. The Cotesia rubecula bracovirus is unique among polydnaviruses in that only four major genes are detected in parasitized
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