A5477
Anti-HA monoclonal
clone HA-7, purified from hybridoma cell culture
Sinónimos:
Anti-HA, Hemaglutinina anti-gripal
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About This Item
Productos recomendados
biological source
mouse
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous glycerol solution
technique(s)
western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins
shipped in
wet ice
storage temp.
2-8°C
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General description
Monoclonal anti-HA, Alkaline Phosphatase conjugate is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a synthetic peptide. Human influenza hemagglutinin (HA), a surface glycoprotein exists as a trimer. The monomeric HA comprises base and membrane distal-tip regions with conserved residues and receptor-binding site, respectively.
Specificity
El anticuerpo reconoce tanto las formas nativas como las desnaturalizadas-reducidas de las proteínas etiquetadas con HA y es reactivo con las proteínas de fusión etiquetadas con HA en N-terminal o C-terminal expresadas en E. coli o en células de mamífero.
Immunogen
péptido sintético correspondiente a un fragmento de la hemaglutinina (HA) del virus de la gripe humana, conocida como etiqueta-HA, conjugado con KLH
Application
Monoclonal Anti-HA−Alkaline Phosphatase antibody produced in mouse has been used in immunoblotting
Biochem/physiol Actions
Human influenza hemagglutinin (HA) is required for the infectivity of the human virus. HA interacts with the host cell surface glycoproteins especially the sialic acid to initiate infection. The short sequence derived from the HA molecule corresponding to amino acids 98-106 (YPYDVPDYA) has been used as a tag, known as HA-Tag. This tag facilitates the detection, isolation, purification, coprecipitation of proteins and immunostaining proteins. Caspase 3/7 effectively cleaves HA-tag. Many recombinant proteins or protein of interest have been engineered to express the HA tag fusion protein. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% BSA, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide as a preservative
Storage and Stability
For continuous use and extended storage, store at 2-8 °C. Do not freeze. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Considerable progress has been made toward understanding the structural basis of the interaction of the two major surface glycoproteins of influenza A virus with their common ligand/substrate: carbohydrate chains terminating in sialic acid. The specificity of virus attachment to target
The HA tag is cleaved and loses immunoreactivity during apoptosis.
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Nature methods, 4(2), 107-108 (2007-02-01)
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