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Merck

45125

Sigma-Aldrich

Elastase from porcine pancreas

lyophilized, powder, white, ~8 U/mg

Sinónimos:

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204

biological source

Porcine pancreas

Quality Level

form

powder

quality

lyophilized

specific activity

~8 U/mg

mol wt

Mr ~25000

impurities

salt, none detected

color

white

storage temp.

−20°C

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Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Other Notes

For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
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Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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I Grunnet et al.
The Biochemical journal, 209(1), 215-222 (1983-01-01)
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides.
T. Sakurai et al.
Journal of the American Chemical Society, 110, 7236-7236 (1988)
Etsuko Kurimoto et al.
Respiratory research, 14, 5-5 (2013-01-22)
Pulmonary emphysema is characterized by alveolar destruction and persistent inflammation of the airways. Although IL-17A contributes to many chronic inflammatory diseases, it's role in the inflammatory response of elastase-induced emphysema remains unclear. In a model of elastase-induced pulmonary emphysema we
Gerwin E Engels et al.
Journal of cardiothoracic surgery, 8, 4-4 (2013-01-11)
Despite continuous improvements in materials and perfusion techniques, cardiac surgery still causes lung injury and a delay of pulmonary recovery. Currently, there is no gold standard for quantifying cardiac surgery induced lung injury and dysfunction. Adding objective measures, such as
Paul D Thornton et al.
Macromolecular rapid communications, 34(3), 257-262 (2013-01-05)
The generation of a range of star-shaped block copolymers composed of a biocompatible poly(ethylene glycol) (PEG) core tethered to a polyalanine (PAla) shell that possesses the capability to (reversibly) self-assemble in water is described. The hydrogels formed offer a hydrophilic

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