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Merck

18470

Sigma-Aldrich

Bromothymol Blue sodium salt

for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6)

Sinónimos:

Bromthymol Blue sodium salt, 3′,3′′-dibromothymolsulfonephthalein sodium salt

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About This Item

Fórmula empírica (notación de Hill):
C27H27Br2NaO5S
Número de CAS:
Peso molecular:
646.36
Beilstein/REAXYS Number:
6887926
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.25

grade

for microscopy (Bot., Hist., Vit.)
for molecular biology
indicator (pH 6.0-7.6)

Quality Level

form

powder

technique(s)

titration: suitable

visual transition interval

6.0-7.6, yellow to blue

storage temp.

room temp

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

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General description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Application

Suitable for use:
  • In preparations of pH indicator solutions
  • In phospholipid staining on tissues and cells prepared on microscopy slides

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Modified bromthymol blue stain for chromatographic detection of phospholipids.
D F Yarmosh et al.
Clinical chemistry, 23(3), 614-615 (1977-03-01)
Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue
Nguyen T D, et al.
Journal of Analytical Methods in Chemistry, 2018 (2018)
Dan Wang et al.
Methods in molecular biology (Clifton, N.J.), 851, 9-46 (2012-02-22)
Pain is a complex sensory experience for which the molecular mechanisms are yet to be fully elucidated. Individual differences in pain sensitivity are mediated by a complex network of multiple gene polymorphisms, physiological and psychological processes, and environmental factors. Here
Haixia Chi et al.
Scientific reports, 10(1), 19581-19581 (2020-11-13)
Zinc tetraphenylporphyrin (Zn-TPP) solubilized by GroEL protein cage was prepared as a supramolecular mimic of carbonic anhydrase (CA) for CO2 capture. It is shown that the soluble Zn-TPP-GroEL complex can be formed easily by detergent dialysis. The Zn-TPP/GroEL binding ratio

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