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Merck

318752

Sigma-Aldrich

Bromothymol Blue sodium salt solution

0.04 wt. % in H2O

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About This Item

Fórmula empírica (notación de Hill):
C27H27Br2NaO5S
Número de CAS:
Peso molecular:
646.36
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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form

liquid

Quality Level

concentration

0.04 wt. % in H2O

pH

6.0-7.6, yellow to blue

density

0.99 g/mL at 25 °C (lit.)

λmax

615 nm

ε (extinction coefficient)

≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

Storage temp.

room temp

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

Inchi Key

NMKFVGALBGZKGW-FKWCIMQXSA-M

General description

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Biochem/physiol Actions

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.[1]

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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R. W. Sabnis
Handbook of Acid-Base Indicators (2007)

Questions

  1. Do you provide dyes or other solutions used for validating adequate cleaning of equipment?

    1 answer
    1. If you are checking for the adequate cleaning of equipment, there are various ways to do so. pH indicators such as bromothymol blue can be used to ensure that alkaline detergents have been washed away. However, dyes are generally not commonly used to check for sterility or whether a surface is clean of all substances. The use of other dyes may vary, and it's essential to consider the specific use and characteristics of each dye for different applications.

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