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Merck

11-0398

Sigma-Aldrich

Fluorescamine

≥98.0%

Sinónimos:

4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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About This Item

Fórmula empírica (notación de Hill):
C17H10O4
Número de CAS:
Peso molecular:
278.26
Beilstein/REAXYS Number:
921143
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:

assay

≥98.0%

availability

available only in Japan

mp

153-157 °C (lit.)

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

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Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Suitability

for HPLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Yusuke Suzuki et al.
Journal of agricultural and food chemistry, 56(22), 10811-10816 (2008-11-06)
Photoinduced decarboxylation via homolytic cleavage of the ester linkage generating two benzyl radicals being recoupled is known to be a major photolytic pathway of the insecticide fenvalerate in aqueous or organic solvents. A highly sensitive and selective fluorescence spectroscopic method
Claire E Stanley et al.
Chimia, 66(3), 88-98 (2012-05-02)
This account highlights some of our recent activities focused on developing microfluidic technologies for application in high-throughput and high-information content chemical and biological analysis. Specifically, we discuss the use of continuous and segmented flow microfluidics for artificial membrane formation, the
Khin Yin Win et al.
The Analyst, 137(10), 2328-2332 (2012-03-13)
Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling
Andrew D Aubrey et al.
Astrobiology, 8(3), 583-595 (2008-08-06)
The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements.
Marcos Mandaji et al.
Electrophoresis, 30(9), 1501-1509 (2009-04-08)
The possibility to compress analyte bands at the beginning of CE runs has many advantages. Analytes at low concentration can be analyzed with high signal-to-noise ratios by using the so-called sample stacking methods. Moreover, sample injections with very narrow initial

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