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Merck

RSTREP-RO

Roche

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

Sinónimos:

streptavidin

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About This Item

Código UNSPSC:
12352202

origen biológico

Streptomyces sp. ( Streptomyces avidinii)

Nivel de calidad

recombinante

expressed in E. coli

Formulario

liquid

actividad específica

≥17 units/mg protein

mol peso

~52 kDa

envase

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

fabricante / nombre comercial

Roche

concentración

70-90%

técnicas

protein array: suitable
protein purification: suitable

impurezas

6-7% water

intervalo de pH

5.0-9.0

pI 

7.4-7.7 (two main bands)

solubilidad

water: 10 mg/L (mA405=100)

idoneidad

suitable for immunological tests

Nº de acceso UniProt

Condiciones de envío

cooler

temp. de almacenamiento

-10 to -25°C

Categorías relacionadas

Descripción general

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Aplicación

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Secuencia

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Definición de unidad

One unit binds 1 μg D-biotin at pH 8.9.

Forma física

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M

Otras notas

For life science research only. Not for use in diagnostic procedures.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash


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Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity
Jouybari RM et al.
Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)
Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
Michael Weber et al.
Nature photonics, 15(5), 361-366 (2021-05-07)
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
Michael Weber et al.
Nature biotechnology, 41(4), 569-576 (2022-11-08)
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the

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