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S0677

Sigma-Aldrich

Estreptavidina from Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

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About This Item

Número de CAS:
9013-20-1
Número MDL:
MFCD00082035
Código UNSPSC:
12352203
NACRES:
NA.46

recombinante

expressed in E. coli

Nivel de calidad

200

Formulario

lyophilized powder

capacidad

4 mol/mol binding capacity (Biotin)

temp. de almacenamiento

−20°C

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Descripción general

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Aplicación

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Acciones bioquímicas o fisiológicas

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Definición de unidad

One unit will bind 1.0 μg biotin.

Forma física

Lyophilized from 5 mM potassium phosphate buffer

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000412936
0000412923
0000412923
0000412936
0000412924

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Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)
The properties of streptavidin, a biotin-binding protein produced by Streptomycetes
Louis C
Archives of Biochemistry and Biophysics, 106 (1964)
Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)
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