MABE331

Anti-MDM2 Antibody, clone 4B2C1.11

clone 4B2C1.11, from mouse

• Sinónimos: E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 4B2C1.11, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_002383
• Nº de acceso UniProt: Q00987
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MDM2(4193) ; mouse ... Mdm2(17246)

Descripción

Descripción general

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Inmunógeno

Recombinant protein corresponding to human MDM2.

Aplicación

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cell lysates (Hjerpe, R., et al. (2010). 42(5):725-735.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cells (Hjerpe, R., et al. (2010). 42(5):725-735.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-MDM2 Antibody, clone 4B2C1.11 (mouse monoclonal antibody) validated in WB, IP, ICC to detect MDM2 also known as E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2.

Calidad

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected MDM2 in 10 µg of mouse brain tissue lysate.

Descripción de destino

~55 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Mouse brain tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable