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MAB2223

Sigma-Aldrich

Anti-DNP Antibody, clone 9H8.1

clone 9H8.1, from mouse

Sinónimos:

Dinitrophenol

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

clon

9H8.1, monoclonal

reactividad de especies (predicha por homología)

all

técnicas

western blot: suitable

isotipo

IgG1κ

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

Dinitrophenol (DNP) is a small water-soluble, hydrophobic ligand that binds to transthyretin at acidic pH and also reduces the formation of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to acclimated bacteria. DNP has been associated with possible therapies for neurodegenerative and neurological disorders.

Especificidad

Demonstrated to react with a DNP moiety in a wider range of species. This antibody′s reactivity is expected to be species independent.

Inmunógeno

KLH-conjugated DNP

Aplicación

This Anti-DNP Antibody, clone 9H8.1 is validated for use in WB for the detection of DNP.

Calidad

Evaluated by Western Blot in Oxyblot protein standards.

Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.

Descripción de destino

Various kDa observed. The Oxyblot protein standards (S7151) were used to test this antibody. These standards are phosphorylase B, BSA, OVA, Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues per protein.

Forma física

Format: Purified

Nota de análisis

Control
Oxyblot protein standards

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Jennifer B M Steffen et al.
The Journal of experimental biology, 226(15) (2023-07-20)
Coastal environments commonly experience fluctuations in salinity and hypoxia-reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood.
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Scientific reports, 14(1), 9658-9658 (2024-04-27)
Sessile benthic organisms like oysters inhabit the intertidal zone, subject to alternating hypoxia and reoxygenation (H/R) episodes during tidal movements, impacting respiratory chain activities and metabolome compositions. We investigated the effects of constant severe hypoxia (90 min at ~ 0% O2 ) followed
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International journal of molecular sciences, 23(9) (2022-05-15)
We investigated the feasibility of detecting the presence of specific autoantibodies against potential tumor-associated peptide antigens by enriching these antibody-peptide complexes using Melon Gel resin and mass spectrometry. Our goal was to find tumor-associated phospho-sites that trigger immunoreactions and raise
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Bone, 158, 116355-116355 (2022-02-14)
Diabetes mellitus (DM) embrace a group of chronic metabolic conditions with a high morbidity, causing deleterious effects in different tissues and organs, including bone. Hyperglycemia seems to be one of the most contributing etiological factors of bone-related alterations, altering metabolic
Brígida R Pinho et al.
Free radical biology & medicine, 146, 372-382 (2019-11-22)
Abnormal protein homeostasis (proteostasis), dysfunctional mitochondria, and aberrant redox signalling are often associated in neurodegenerative disorders, such as Huntington's (HD), Alzheimer's and Parkinson's diseases. It remains incompletely understood, however, how changes in redox signalling affect proteostasis mechanisms, including protein degradation

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