Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).
Immunogen
Bovine
purified poly(ADP-ribose)
Application
Immunoblotting (2.5 µg/ml)
Immunocytochemistry (5-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control Normal mouse IgG
Positive Control Etoposide-treated HL-60 cells
Other Notes
Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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